Coding

Part:BBa_K593007

Designed by: Ceren Seref   Group: iGEM11_METU-Ankara   (2011-09-21)

mmo B

mmo B gene encodes protein B of soluble methane monooxygenase from Methylococcus capsulatus (Bath).This gene encodes approximately 16kDa protein B.At low concentrations, Protein B converts the hydroxylase from an oxidase to a hydroxylase and stabilises intermediates necessary for oxygen activation. Saturating amounts of Protein B dramatically increase the rates of formation of intermediates and accelerate catalysis of methane to methanol by soluble methane monooxygenase.sMMO is comprised of three components; A, B and C. Protein C (the reductase) transfers electrons from NADH to protein A (the hydroxylase) which contains the active site, and protein B regulates this electron flow.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None